Plasmid_Backbone

Part:BBa_K3830009

Designed by: Fang Ti   Group: iGEM21_NCHU_Taichung   (2021-10-18)


origin of replication

F1 is a phage-derived ori that allows for the replication. The origin of DNA

replication of bacteriophage f1 functions as a signal. It can enhance the synthesis of the

downstream genes

T--NCHU_Taichung--NCHU_2021_Figure_AB.png

Figure. The schematic diagram of f1 ori


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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